Does the time difference between Istanbul and Madrid change during the year?

Yes. Because of daylight saving time the gap moves between +1h and +2h.

When is the best time to call Madrid from Istanbul?

Between 10:00 and 17:00 in Istanbul, which is 09:00 to 16:00 in Madrid.

Istanbul is 1h ahead of Madrid. This gap is not fixed: daylight saving time moves it between +1h and +2h during the year.

Istanbul

Türkiye

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Madrid

Spain

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A 9-to-5 day in each city overlaps for 7h. That is 10:00–17:00 in Istanbul, which is 09:00–16:00 in Madrid.

City	Time zone	UTC offset
Istanbul · Türkiye	Europe/Istanbul	UTC+3
Madrid · Spain	Europe/Madrid	UTC+2

What is the time difference between Istanbul and Madrid?: Istanbul is 1h ahead of Madrid. This gap is not fixed: daylight saving time moves it between +1h and +2h during the year.
Does the time difference between Istanbul and Madrid change during the year?: Yes. Because of daylight saving time the gap moves between +1h and +2h.
When is the best time to call Madrid from Istanbul?: Between 10:00 and 17:00 in Istanbul, which is 09:00 to 16:00 in Madrid.